Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
نویسندگان
چکیده
منابع مشابه
Detection of Ostreid Herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus 1 (OsHV-1) detectio...
متن کاملRapid and sensitive detection of ostreid herpesvirus 1 in oyster samples by real-time PCR.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and ...
متن کاملExperimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: kinetics of virus DNA detection by q-PCR in seawater and in oyster samples.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transm...
متن کاملViruses infecting bivalve molluscs
Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 and 1973) were...
متن کاملSaxitoxin analysis in bivalve molluscs
Paralytic shellfish poisoning (PSP) is caused by consumption of a wide variety of shellfish which accumulated saxitoxins (STXs) from marine dinoflagellates (Alexandrium minutum, A. tamarense, Gymnodinium catenatum, Pyrodinium bahamense) and affect a wide variety of shellfish. There are about 20 saxitoxin analogs with closely related structures. A regulatory level of 0.8 mg/kg shellfish meat as ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2007
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2006.09.005